转AhCMO基因棉花品系苗期耐盐性的比较研究

Comparative Study on Salt-tolerance of Transgenic AhCMO Cotton Seedlings

以转AhCMO基因的5个棉花品系(CMO1、CMO2、CMO3、CMO4、CMO5)及其转化受体泗棉3号(SM3)为材料,在日光温室盆栽条件下,比较研究了转AhCMO基因棉花品系耐盐胁迫能力的差异。试验分3次加入5%的NaC l溶液,使土壤含盐量达到0.5%(土壤水分含盐量,W/W)为盐处理,以不加NaC l的处理为对照。结果表明,盐胁迫后,转化受体和所有转基因品系的生长发育都受到显著抑制。但5个转基因品系在发芽率、株高、真叶数、鲜重、光合参数、荧光参数等方面均有明显提高或改善,同时转基因品系之间也存在显著差异,CMO2、CMO4相对于其它转基因品系表现出更强的耐盐性。

Five transgenic AhCMO cotton lines （CMO1, CMO2, CMO3, CMO4 and CMO5） and their receptor Simian 3 （SM3） were grown in pots in a greenhouse, and their tolerance to salinity were comparatively studied. Pots were drenched with 5% NaCl solution for 3 times until the final NaCl concentration in each pot reached 0.5% （based on soil moisture, W/W） as salt-stressed treatment, and pots with distilled water as their corresponding controls. The results showed that the growth and development of all transgenic lines and their receptor were significantly inhibited, but germination rate, plant height, the number of true leaves, flesh weight, photosynthetic parameters, and fluorescence parameters of the five transgenic lines were significantly raised or improved. There were significant differences in salt tolerance among transgenic lines. CMO2 and CMO4 possessed higher salt tolerance than other transgenic lines.