纳米荧光染料C-dots标记细胞的应用

Application of silica nanoparticles C-dots in fluorescent labeling for cells

目的探讨一种新型纳米荧光染料C-dots作为细胞标记物的应用。方法不同直径的C-dots和B16小鼠黑色素瘤细胞共同培养,用激光共聚焦荧光显微镜观察C-dots被摄取及在细胞内分布情况。利用MTT比色法测定C-dots标记的细胞及未标记细胞的增殖情况;用台盼蓝染色法检查标记细胞活性。结果直径10 nm和5 nm C-dots可用于标记细胞。在共聚焦显微镜下,可见C-dots标记细胞的细胞膜带有近红外荧光。利用MTT比色法测定标记及未标记细胞的A值无显著差异,C-dots标记细胞的活性与对照组相比也无显著差异。结论C-dots可作为细胞内的标记物,用于细胞示踪、增殖试验等研究。

Objective To explore the application of near infrared fluorescent dye C-dots in cell labeling. Methods B16 melanoma cells were cultured with C-dots of various diameters. The distribution of C-dots in cells was observed by laser confocal fluorescent microscope. The proliferation of both C-dots-labeled and unlabeled cells were measured by MTI＂ colorimetry. The viability of labeled cells was checked by trypan blue staining. Results C-dots with diameter of 5 or 10 nanometers can be used to label cells. The near infrared fluorescence of C- dots was taken into the cells and observed on the cellular membrane. No difference of cellular viability was found between the labeled and unlabeled cells. Conclusion C-dots can be used as an intracellular marker for the research on trace and proliferation of cells.