慢性丙型肝炎患者外周血CD5^+ B细胞检测及意义

Detection and of CD5-positive B cells in peripheral blood mononuclear cells of patients with chronic HCV infection and its clinical significance

目的研究慢性丙型肝炎(CHC)患者外周血CD5+B细胞的水平及其临床意义。方法采用流式细胞术检测CHC患者外周血B细胞表面CD5分子的表达;应用荧光定量PCR检测HCV RNA滴度,并与肝功能指标ALT进行相关性分析。结果CHC患者外周血CD5+B细胞显著增多,达(58.4±9.8)%,显著高于慢性乙型肝炎组(39.8±8.2)%和正常对照组(22.5±5.9)%(P均小于0.01)。其与ALT无显著相关性(r=0.2,P>0.05),而与HCV RNA拷贝数呈显著正相关(r=0.817,P<0.05)。结论CD5+B细胞可能参与HCV感染的慢性化过程。

Objective To investigate the percentage of CDS-positive B cells in peripheral blood mononuclear cells （PBMC） of patients with chronic HCV infection and its clinical significance. Methods The expression of CD5 molecule on B cell surface was detected by flow cytometry and HCV RNA copies were detected by real-time PCR. Results The percentage of CD5 ^＋ -B ceils significantly increased in the patients with chronic HCV infection （58.4% ± 9.8% ） compared with healthy controls （22.5% ± 5.9% ） （ P 〈 0. 01 ） and chronic HBV infection （39. 8% ± 8.2% ） （P 〈 0. 01 ）. The expansion of CD5 ~ -B cell was positively correlated with HCV viral load. Conclusion The expansion of CD5 ^＋ -B ceils in the peripheral blood of HCV-infected patients may play a possible role in development of HCV-associated autoimmunity.