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半乳凝素-10在CD4^+ CD25^+ CD127^(low/-)调节性T细胞中的表达与意义 被引量：1

Expression of galectin-10 in CD4^+ CD25^+ CD127^(low/-) regulatory T cells
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摘要 目的探讨半乳凝素-10(galectin-10)在CD4+CD25+CD127low/-调节性T细胞(regulatory T cell,Treg)中的表达及其与Foxp3的相关性。方法通过流式细胞术分选健康人外周血和胃癌患者癌旁淋巴结中Treg细胞与CD4+CD25-CD127+T细胞,经微量抽提RNA后,用RT-PCR检测galectin-10和Foxp3的mRNA表达水平。结果galectin-10和Foxp3的mRNA显著表达于Treg细胞,几乎不表达于CD4+CD25-CD127+T细胞。健康人外周血和胃癌患者癌旁淋巴结中的结果一致。结论ga-lectin-10基因高表达于Treg细胞中,可以作为Treg细胞一项新的标志物。 Objective To investigate the expression of galectin-10 in CD4^＋CD25^＋CD127^low/- regulatory T cells （Treg） and the correlation between expressions of galectin-10 and Foxp3. Methods CD4＋ CD25 ^＋ CD127^low/- Treg cells and CD4^＋ CD25 ^- CD127 ^＋ T cells were isolated from PBMC of healthy volunteers and lymph nodes of gastric cancer patients by flow cytometry. Following RNA micro-extraction RT-PCR analysis was used to detect mRNA expression of galectin-10 and Foxp3. Results The mRNA expression of galectin-10 and Foxp3 were dominant in Treg cells, whereas nearly no expression in CD4 ^＋ CD25^- CD127^ ＋ T cells. The results from PBMC of healthy volunteers and lymph nodes of gastric cancer patients were consistent between. Conclusion The expression of galectin-10 was obviously high in Treg cells, so it may become a novel marker for phenotypic characterization of Treg ceils.
作者 王剑 袁向亮 薛剑 李美星 沈立松
机构地区 上海交通大学医学院附属新华医院检验科
出处 《临床检验杂志》 CAS CSCD 北大核心 2008年第5期365-367,共3页 Chinese Journal of Clinical Laboratory Science
关键词 半乳凝素CD4^+CD25^+CD127^low/-调节性T细胞 Foxp3 细胞分选 galectin-10 CD4^＋CD25^＋CD127^low／- regulatory T cells Foxp3 cell sorting
分类号 R446.63 [医药卫生—诊断学]
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参考文献9

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共引文献19

同被引文献30

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临床检验杂志
2008年 第5期

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