离子束处理黑麦草对水体中硝基苯的去除及其生理响应

NITROBENZENE REMOVAL FROM AQUEOUS SOLUTIONS BY PERENNIAL RYEGRASS WITH N^+ ION IMPLANTATION AND PHYSIOLOGICAL RESPONSES

在室内水培实验条件下,研究了离子束辐照处理的黑麦草对水体中硝基苯的消除能力及其生理响应.结果表明:在25keV,2.6×1016N+·cm-2注入处理下,黑麦草植株生长良好,对硝基苯的去除效率高于对照,且硝基苯的去除动力学符合方程y=51.20-4.77t+0.13t2(R=0.994);在25keV,3.9×1016N+·cm-2注入处理下,植株生长正常,对硝基苯的去除效率略低于对照.进一步分析离子束处理黑麦草在硝基苯胁迫下的生理响应发现,3.9×1016N+·cm-2注入剂量下,黑麦草丙二醛(MDA)的含量呈现先降低后升高再降低的变化趋势,2.6×1016N+·cm-2注入剂量下,黑麦草MDA的含量呈现先升高后降低再升高的变化趋势;3.9×1016N+·cm-2注入剂量下,黑麦草过氧化物酶(POD)活性相对较高,表现出先升高后降低再升高的变化趋势,2.6×1016N+·cm-2注入处理剂量下,POD酶活性总体上高于对照;两种剂量处理条件下,过氧化氢酶(CAT)活性的变化均表现出先升高后降低再升高的趋势.这说明适当能量剂量的N+离子束辐照在一定程度上能够提高黑麦草对硝基苯的耐受去除能力.

The effect on nitrobenzene remove by perennial ryegrass （Lolium perenne） with N^＋ ion implantation was studied. The experiment results showed that at the energy of 25 keV, when the dose was 2.6× 10^16 N^＋· cm^-2, all of the plants grew well, the removal efficiency of nitrobenzene was better than those of the controls. While the dose was 3.9 × 10^16 N^＋· cm^-2, the removal efficiency was worse. The dynamic test results were fitted well by y =A ＋A1t ＋A2t2, and the relativity coefficients were above 0. 994. The experiment results of physiological responses of L. perenne under nitrobenzene showed that the change tendency of malon- dialdehyde （MDA） contents of L. perenne with N^＋ ion implantation decreased at first, then increased, after that it also decreased. The peroxidase （POD） activity change of L. perenne with 3.9× 10^16× N^＋· cm^-2ion implantation was similar with the controls, but L. perenne with 2.6 × 10^16 N^＋· cm^-2 ion implantation was different from the controls. The catalase （CAT） activities of L. perenne with N ＋ ion implantation increased at first, then decreased, and increased again. This primarily indicated that proper energy and dose of N^＋ ion beam irradiation was useful in improving the tolerance and removal of L. perenne to nitrobenzene.