猪链球菌2型对PK-15细胞的黏附动力学

Adhesion Kinesics of Streptococcus suis Type 2 to PK-15 Cells

为研究携带不同毒力因子的猪链球菌2型对细胞侵袭作用的差异,本实验室从全国各地分离100多株猪链球菌,PCR鉴定出10株链球菌2型,其中携带毒力因子菌株为7-4[MRP+EF+]、8-3[MRP+EF-]、ZH-1[MRP-EF+],另外选择1株LN-2[MRP-EF-]与PK-15细胞进行黏附动力学试验,结果表明MPR毒力因子与菌株对PK-15细胞黏附具有密切相关性。

The objective of this paper was to study the different invasiveness among streptococcus suis type 2 which carried various virulence factors, 100 strains of S suis were isolated from all over the country. 10 strains of them were S suis type 2 by PCR identification, strains with virulence factors were selected to explore the adhevence of them to PK-15 cells. 7 4[-MRP＋ EF＋ ] ,8-3[-MRP＋ EF- ] ,ZH-1EMRP- EF＋ ], and control strains LN-2 [MRP- EF ] were chose in adhesion kinesics test to PK-15 cells. The results indicated that MRP factor was closely related to adhesion of S suis type 2 to PK-15 cells.