期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

RNA病毒感染性分子克隆的研究

Study on Infectious Molecular Clone of RNA Virus
下载PDF
导出
摘要 随着反向遗传学技术的兴起和发展,RNA分子体外操作已经成为可能,并且技术日臻完善。反向遗传操作技术的关键就是构建RNA病毒的感染性分子克隆,在DNA分子水平上对其进行体外操作,从而研究病毒结构与功能的方法。作者对感染性克隆的构建方法及感染性体外转录方法作一综述。 Following the rise and developmemt of reverse genetics technique, it become possible to work with RNA mole cule in vitro. The construction infectious molecular clone of RNA virus and operation in DNA the key to reverse genetics technique. Thevirus＇ ~s structure and function can be studied by this way. constructing infectious clone and in vitro transcription were reviewed.
作者 钱淑凤 郝玉兰 郑翠玲
机构地区 唐山职业技术学院环境工程系
出处 《中国畜牧兽医》 CAS 2008年第9期34-37,共4页 China Animal Husbandry & Veterinary Medicine
关键词 RNA病毒 感染性克隆 感染性体外转录本 RNA virus infectious clone infectious in vitro transcription
分类号 Q785 [生物学—分子生物学]
  • 相关文献

参考文献14

  • 1陈稚峰,张立国,董婕,吴昆昱,陈爱珺,孙梅生,黄银霞,齐立,张智清,郭元吉.应用反向遗传学技术在哺乳动物细胞中产生甲型流感病毒[J].病毒学报,2002,18(3):193-197. 被引量：22
  • 2赵恩茂,宋卫兰.动脉炎病毒属病毒的传染性cDNA克隆及其作为疫苗载体的研究进展[J].中国畜牧兽医,2006,33(10). 被引量：2
  • 3韩剑峰,秦鄂德.痘苗病毒载体在冠状病毒反向遗传学中的应用[J].国外医学（病毒学分册）,2005,12(4):121-125. 被引量：1
  • 4Casais R, Thiel V. Reverse genetics system for the avian coronavirus infectious bronchitis virus. Virol, 2001 , 75(24) : 12359-12369.
  • 5Coley S E, Lavi E, et al. Recombinant mouse hepatitis virus strain A59 from cloned, full-length cDNA replicates to high titersin vitro and is fully pathogenic in vivo. J ViroI, 2005,79(5) : 3097-3106.
  • 6Guan Y, Zheng B J, He Y Q, et al. Isolation and characterization of viruses related to the SARS corona-virus from animals in southern China. Science, 2003,302 : 227- 278.
  • 7Martina B E, Haagmans B L, Kuiken T, et al. SARS virus infection of cats and ferrets. Nature,2003, 425: 915.
  • 8Neumann G, Whitt M A ,Kawaoka Y. A decade after the generation of a negative-sense RNA virus from cloned cDNA what have we learned. J Gen Virol,2002,83:2635-2662.
  • 9Rota P A, Oberste M S,Monroe S S, et al. Characterization of a noveleoronavirus associated with severe acute respiratorysyndrome. Science, 2003,300: 1394-1399.
  • 10Snijder E J, Bredenbeek P J, Dobbe J C, et al. Unique and conserved features of genome and proteome of SARS-eoronavirus, an early splitoff from the coronavirus group lineage. J Mol Biol, 2003,331:999-1004.

二级参考文献33

  • 1出版社吉 程小雯.红细胞凝集及凝集抑制试验.流行性感冒病毒及其实验技术[M].北京:中国三峡出版社,1997.100-106.
  • 2Holmes, K.V. Coronaviruses. In: Knipe, D.M, Howley, P.M,et al, editors. Fields Virology, 4th edition. Philadelphia: Lippincott Williams & Wilkins; 2001. p. 1187-1203.
  • 3Masters, P. S, Rottier, P.J. Coronavirus reverse genetics by targeted RNA recombination. Curr Top Microbiol Immunol, 2005,287:133-159.
  • 4Almazan, F, Gonzalez,J. M, et al. Engineering the largest RNA virus genome as an infectious bacterial artificial chromosome. Proc.Natl. Acad. Sci. USA, 2000, 97(10):5516-5521..
  • 5Thiel,V, Herold,J, et al. Infectious RNA transcribed in vitro from a cDNA copy of the human coronavirus genome cloned in vaccinia virus. J. Gen. Virol, 2001,82:1273-1281.
  • 6Yount,B, Curtis, K. M, et al. Strategy for systematic assembly of large RNA and DNA genomes: transmissible gastroenteritis virus model. J. Virol, 2000,74(22): 10600-10611.
  • 7Yount,B, Denison,M. R, et al. Systematic assembly of a full-length infectious cDNA of mouse hepatitis virus strain A59. J. Virol, 2002,76(21): 11065-11078.
  • 8Yount, B, Curtis, K. M, et al. Reverse genetics with a full-length infectious cDNA of severe acute respiratory syndrome coronarins.Proc. Natl. Acad. Sci. USA, 2003, 100:12995-13000.
  • 9Casais,R, Thiel,V, et al.Reverse genevics system for the avian coronavirus infectious bronchitis virus. J. Virol, 2001,75 (24):12359-12369.
  • 10Coley, S. E, Lavi, E, et al. Recombinant mouse hepatitis virus strain A59 from cloned, full-length cDNA replicates to high titers in vitro and is fully pathogenic in vivo. J Virol,2005,79(5) :3097-3106.

共引文献22

中国畜牧兽医
2008年 第9期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部