水貂自咬症病因的分子遗传研究

The Molecular Genetic Study on Etiological Factor of Mink Self-Biting

自咬症是笼养毛皮动物水貂、狐狸的常见病,严重影响着毛皮质量,然而其发病原因一直未得以明确。作者初步从分子遗传学角度探讨了水貂自咬症的发病原因。采用随机扩增多态DNA(RAPD)技术分别对正常水貂组和自咬水貂组进行了遗传基因差异上的比对分析。从100个随机引物中筛选出40个重复性好的多态引物,提取水貂健康与患病群体DNA各40个,随机抽取10个构建基因池,进行RAPD标记研究。挑选出在健康组与患病组差异明显的S103引物(序列为AGACGTCCAC)并对其进行克隆,将克隆结果交上海生工生物有限公司测序。引物S103扩增出健康与患病水貂的特异条带,仅在患病水貂组中发现750bp左右的DNA片段,而在健康组中发现600bp左右的特异片段。此两条特异片段可初步作为区分健康和患病水貂群体的分子遗传标记,为进一步对水貂自咬症的深入研究奠定了基础。

Self-biting is the frequent diease to furriery animal such as mink,fox,which affect on the furriery qualities. The reason isn＇t definite. Primarily discuss the reason from molecule genetics. Constraet the group of health and self-biting with RAPD （random amplified polymorphic DNA,RAPD）,randomly select 40 polymorphisms primers which are good reproducibili- ty. Abstract 40 DNA from each group, and random construction gene cistern from 10 DNA. Primer S103 （sequence is AGACGTCCAC） with obviouslily difference from health and selgbiting is selected, and clone, send the result of clone to Sangon biologic company in shanghai to sequence. Through primer S103, only in the disease mink groups we amplify 750 bp DNA fragment, 600 bp DNA is showed up in the health group but disease group isn＇t. So this can serve as molecular genetic labeling to distinguish from healthy and sick mink groups. So the different fragments both primarily distinguish the molecular genetic labe- ling from health and diease,it bases on the deeply study for the self-biting.