Effects of Different Culture Conditions on Vitrification of Lycium barbarum L. Plantlets in Tissue Culture

不同培养条件对枸杞组培苗玻璃化的影响(英文)

The tender stems from new Lycium barbarum L. cultivar ＂Ningqi 3＂ released by Ningxia Academy of Agricultural and Forestry Sciences were regarded as explants to investigate the vitrification of Lycium barbarum plantlets in tissue culture under different concentrations of 6-BA, sucrose, agrose, culture temperature, and illumination duration with MS as basic medium. The results show that the conditions for maximal proliferation coefficient and min- imal vitrification are as following： the basic medium with 0.2 mg/L 6-BA, 3% sucrose and 0.65% agarose; culture at 25℃; 12 h/d（ daylight lamp, 2 000 lx） illumination.

笔者对枸杞玻璃化现象的影响因素进行了研究，以期为预防枸杞玻璃化苗的产生和提高枸杞组培苗的质量提供有效措施。