摘要
[ Objective] The aim of this study is to obtain transgenic Lilium longiflorum Thumb. [ Method] A two-step method of explant and the T-DNA integration technique were employed to transform Lilium longiflorum via Agrobacterium mediated method. [ Result] The best infection effect appeared under the OD600 value of Agrobacterium within 0.6 -0.8, the addition of 250 mg/L AS could increase the transformation efficiency. The optimal concentration of G418 for screening is 50 mg/L. Some putative transgenic plants of Lilium longiflorum with resistance to G418 showed positive in PCR, preliminarily proving that T-DNA gene had integrated into the genome of lily. [ Conclusion] The study may lay a foundation for breeding excellent lily varieties through TDNA integration technique.
采用2步外植体法结合比较成熟的农杆菌介导T—DNA插入技术转化麝香百合，为快速选育百合新品种奠定基础。
基金
the Fund of Basis Scientific Research Operation of Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences
the Grant of Scientific Fund of Chinese Academy of Tropical Agricultural Sciences (NoRky0529)~~
