壳寡糖铕、铽配合物的合成、表征及抗·O_2^-活性

Synthesis,Characterization and ·O_2^- Scavenging Activity of Coordination Compounds of Chitooligosaccharide with Eu^(3+) and Tb^(3+)

用壳寡糖分别与硝酸铕和硝酸铽反应,制备了壳寡糖-铕、壳寡糖-铽两种配合物。用红外光谱、紫外光谱、荧光和X射线光电子能谱(XPS)等分析测试手段对配合物进行了表征。以吩嗪硫酸甲酯(PMS)-还原型辅酶Ⅰ烟酰胺腺嘌呤二核苷酸(NADH)-硝基四氮唑蓝(NBT)产生超氧阴离子自由基(.O2-)来研究壳寡糖和壳寡糖稀土金属配合物对.O2-自由基的清除作用。结果表明:壳寡糖与Eu3+或Tb3+形成了配合物,壳寡糖-铕、壳寡糖-铽配合物中不仅壳寡糖氨基上的N原子参与了配位,同时仲羟基的O原子也参与了配位。壳寡糖和壳寡糖稀土金属配合物对.O2-均具有明显的清除作用,配合物与壳寡糖相比对.O2-具有更高的清除活性。

The coordination compounds were synthesized from chitooligosaccharide with Eu （NO3）3 and Tb（NO3）3, and were characterized by IR, UV-vis, fluorescence, and X-ray photoelectron spectroscopy, respectively. The scavenging effects of chitooligosaccharide and the rare earth complexes on superoxide anion free radical by NADH-PMS-NBT system were studied. The results showed that Eu3^＋ ion or Tb^3 ＋ ionwas coordinated with amino groups and secondary hydroxyl groups of chitooligosaccharide. The chitooligosaccharide and its complexes all had significant scavenging effects on the superoxide anion free radical. The rare earth complexes displayed the highest scavenging effects on the superoxide anion free radical.