摘要
目的研究亚慢性铝染毒对断乳大鼠神经行为学指标和脑脂质过氧化的影响,以探讨铝对发育中大鼠中枢神经系统的毒性作用及其机制。方法将初断乳清洁级 Wistar 大鼠随机分为对照组(蒸馏水)、低剂量组(2 g/L AlCl_3溶液)、高剂量组(4 g/L AlCl_3溶液),每组10只,自由饮水摄入 AlCl_3,连续染毒12周。染毒后,称量各组大鼠体重和脑组织重量,计算脑组织系数,采用原子吸收石墨炉法测定血铝含量,采用跳台试验法观察大鼠学习、记忆行为学的改变.采用氧化损伤试剂盒测定脂质过氧化水平[丙二醛(MDA)含量、还原型谷胱甘肽(GSH)含量和超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活力]。结果高剂量组大鼠脑组织系数低于对照组,差异有统计学意义(P<0.05)。随着染铝剂量的增加,血铝水平呈上升趋势(P<0.01)。跳台试验结果显示,随铝暴露剂量的增加,大鼠的潜伏期逐渐缩短,而错误次数逐渐增加,有明显的剂量-效应关系(P<0.01)。随着染铝剂量的增加,脑组织中 MDA 含量显著升高(P<0.01),SOD 活力下降;GSH 含量和 GSH-Px 活力先上升后降低。不同组别雌雄大鼠间各指标比较,差异均无统计学意义(P>0.05)。结论亚慢性铝暴露可以引起发育中大鼠学习记忆能力下降,脑脂质过氧化水平提高。
Objective To explore the effect of sub-chronic aluminium exposure on neurological behavior and lipid peroxidation of brain in weaning rats in order to find the mechanism how aluminium impacts CNS development. Methods Thirty weaning Wistar rats were randomly divided into three groups: control and 2 experimental groups which were administrated by aluminium chloride in drinking water at the doses of 0, 2 g/L, 4 g/L respectively for 12 consecutive weeks. The concentration of aluminium in blood was determined by atomic absorption spectrophotometry (AAS). The neurological behavior including learning and memory were detected by platform experiment. The level of lipid peroxidation was determined by the kits including the content of MDA and GSH, the activities of SOD and GSH-Px. Results The coefficient of the brain increased in the high dose group compared with the control group (P〈0.05).The blood aluminium level increased significantly as the treatment dose increased (P〈 0.01). The latent period shortened while mistake times increased significantly, with a association to the dose of aluminium(P〈0.01). The concentrations of MDA increased and the activity of SOD decreased no significantly, the levels of GSH decreased remarkably and the activity of GSH-Px decreased significantly (P〈0.01) as the dose of aluminium increased. Conclusion Sub-chronic aluminium exposure can impair the capacity of learning and memory in rats in developing and can enhance the lipid peroxidation in the brain.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2008年第9期767-769,共3页
Journal of Environment and Health
基金
国家自然科学基金资助项目(30371229)
关键词
铝
学习
记忆
脂质过氧化
Aluminium
Learning
Memory
Lipid peroxidation