N-乙酰半胱氨酸和α-硫辛酸对烹饪油烟凝集物氧化损伤的拮抗作用

Antagonistic Effect of N-acetyI-L-cysteine and α-Lipoic Acid to Oxidative Damage in Liver and Kidneys of Rats Induced by Condensate of Cooking Oil Fume

目的探讨 N-乙酰半胱氨酸(N-acetyl-L-cysteine,NAC)和α-硫辛酸(lipoic acid,LA)对烹饪油烟凝集物(condensates of cooking oil fumes,COF)染毒大鼠肝细胞和肾皮质细胞产生的氧化损伤的拮抗作用。方法将 SPF 级 SD 雄性健康大鼠随机分为4组,每组8只,即阴性对照组、染毒组、NAC 干预组(腹腔注射2 mmol/kg 的 NAC 溶液)和 LA 干预组(腹腔注射0.35 mmol/kg 的 LA 溶液)。6 h 后,将对照组皮下注射同量的生理盐水,其他3组皮下注射10 ml/kg 的 COF。染毒后48 h,测量大鼠肾皮质细胞和肝细胞 SOD、GSH-Px 活力和 MDA 含量。结果与阴性对照组比较,COF 组大鼠肾皮质细胞和肝细胞 SOD、GSH-Px 活力降低(P<0.05和 P<0.01),MDA 含量升高(P<0.05和 P<0.01)。与 COF 组比较,对于大鼠肾皮质细胞,NAC 干预使 SOD、GSH-Px 活力明显增高(P<0.01),MDA 含量显著降低(P<0.05);LA 干预使 SOD、GSH-Px 活力明显增高(P<0.01),MDA 含量显著降低(P<0.01)。与 COF 组比较,对于大鼠肝细胞,NAC 干预使 SOD、GSH-Px 活力明显增高(P<0.05和 P<0.01),MDA 含量显著降低(P<0.01);LA 干预使 SOD、GSH-Px 活力明显增高(P<0.05和 P<0.01),MDA含量显著降低(P<0.01)。结论 NAC 和 LA 对 COF 染毒大鼠肝、肾皮质细胞产生的氧化损伤存在着显著的拮抗作用。

Objective To study the antagonistic effect of N-acetyl-L-cysteine （NAC） and α-lipoic acid （LA） to the oxidative damage in the liver and kidney of rats induced by the condensate of cooking oil fume （COF）. Methods SD rats were randomly divided into 4 groups, 8 in each, the blank control, COF group （10 ml/kg, subcutaneous injection ）, COF plus NAC （2 mmol/kg, intraperitoneal injection ） and COF plus LA （0.35 mmol/kg, intraperitoneal injection）. Forty-eight hours after treatment, the activity of SOD, GSH-Px and MDA level in the livers and kidneys were determined. Results In the kidney, compared with the COF group, both of NAC and LA could increase the activity of kidney SOD and GSH-Px and decreased the MDA level significantly, the same results were seen in the liver. Conclusion Both of N-acetyl-L-cysteine and α-lipoic acid has an obvious antagonistic effect to the oxidative damage in the liver and kidney of rats induced by the condensates of cooking oil fume.