KAI1在甲状腺乳头状癌中的表达及其临床意义

Expression of KAI1 in papillary thyroid cancer and its clinicopathological significance

目的:研究肿瘤转移抑制基因KAI1在甲状腺乳头状癌(PTC)细胞膜和细胞浆中的表达有无差异;KAI1在PTC中表达情况的临床意义。方法:Western blot法分别检测:KAI1蛋白在30例PTC原发灶标本、7例淋巴结转移灶标本、13例癌旁组织标本和13例良性病变标本细胞膜和细胞浆中的表达;KAI1在30例PTC原发灶中总蛋白表达情况。结果:KAI1蛋白在PTC标本胞膜上表达明显低于胞浆中;而在癌旁组织、淋巴结转移标本和良性病变中表达无差异。KAI1蛋白在PTC中的表达阳性率与有无淋巴结转移有明显相关,无淋巴结转移者阳转移性率明显高于有淋巴结转移者;而KAI1表达阳性率与肿瘤原发灶大小及患者年龄无关、与TNM分期负相关。结论:KAI1蛋白在肿瘤组织中表达没有定位于细胞膜可能是其失去对肿瘤进展和转移抑制的原因。KAI1蛋白的低表达对PTC的临床进程明显相关,可作为判断临床演进、淋巴转移的指标和潜在的治疗靶向。

Objective：To explore the expression of tumor metastasis suppressor KAI1 in membrane and cytoplasm of papillary thyroid cancer （PTC） cell; and to explore the elinieopathologieal signifieance of total expression of KAI1 protein in PTC. Methods：we used western blot to investigate：the KAI1 protein expression in membrane and eytoplasm in 30 primary PTC cases, 13 cases of tissue near the cancer,7 eases of lymph node metastases and 13 benign eases ;total expiessions of KAI1 protein in 30 PTC eases. Results： the expression of KAI1 protein in membrane was significant lower than that in eytoplasm of primary PTC ;while the expression were unchanged in membrane and cytoplasm of tissues near the cancer,lymph ＇node metastases and benign cases. The positive expression of KAI1 protein was correlated with the absence of lymph node metastases and the low stage of TNM;there were no significant correlation between KAI1 expression and primary tumor size or patient age. Conclusion：Loss of loeation in cell membrane may be the cause of KAI1 losing its ability to suppress the tumor progression and metastases. The low expression of KAI1 protein was significantly correlated with the clinical progression and lymph node metastases of PTC and it may be used to anticipate the progression and metastases, and may be the potential target of PTC for therapy.