摘要
利用饱和酚法提取了棉纤维发育突变体胚珠的全蛋白以及种子水溶性蛋白,比较分析了不同发育时期棉花胚珠蛋白含量的变化。通过对野生型和纤维突变体全蛋白的SDS凝胶电泳图谱的比较分析,在二倍体棉种的突变体及其野生型间找到了三条可能与表皮细胞分化相关的差异蛋白条带,在种子水溶性蛋白中则发现了五条差异条带,为进一步研究与棉纤维分化相关的分子机制提供了参考。
The whole proteins of ovules and water-soluble proteins from seed in cotton fiber mutant were extracted and the variation of the protein contents of cotton ovules during different growing stage were analyzed. Through comparing the SDS-PAGE patterns between the wild types and their mutants, three distinct bands which are probably relatived to the initiation of the ovule epidermal cell have been found between the mutant and its wild type of the diploid cotton. Moreover, we also discovered five distinct bands in the wild type and its mutant of the species from SDS-PAGE profile of seed water-soluble proteins. This research provides some information for further detecting the molecular mechanics about the differentiation and development of cotton fiber.
出处
《棉花学报》
CSCD
北大核心
2008年第5期330-334,共5页
Cotton Science
基金
国家973计划(2004CB117301)
