基于微电极阵列的微生物电融合

Microelectrode Arrays for Microbial Electrofusion

利用自主研制的梳状交叉微电极阵列细胞电融合芯片系统,研究微生物电融合。在酶解浓度1.5%、酶解温度33℃、酶解时间3h、酶解pH值6.0、0.8mol/L山梨醇的渗透压条件下,获得生成率和再生率分别为95.2%和8.9%的微生物细胞原生质体。在脉冲峰值电压50V、持续时间80μs、8个脉冲、间隔1s的电融合条件下,该原生质体通过电融合获得一株菌株其乳化性能从62%提高到85%。

Microbial electrofusion was studied with an electrofusion system,which was developed based on an interdigital and pectinate microelectrode arrays. The results showed that the formation rate of protoplasts was 95.2% and generation rate was 8.9% at the optimum conditions of 1.5% snailase at 33℃ for 3h,pH 6.0,and 0.8mol/L D-sorbitol as osmotic stabilizer. A new strain was obtained through electrofusion system at intensity of 50V,8 electrofusion pluses and interval of 80μs. Compared with parents,the emulsibility of the new was increased from 62% to 85%.