SPME萃取头对茶油挥发物萃取效果的影响

Effects of SPME Fibers on Volatile Extraction of Camellia Oil

固相微萃取目前被广泛地用来测定液态、气态,甚至固态物质的挥发组分,不同待测物的性质决定了萃取头的种类选择。采用4种常用的SPME涂层萃取头(100μmPDMS、85μmPA、65μmPDMS/DVB、50/30μm DVB/CAR/PDMS)对茶油挥发物的提取性能进行了比较研究,结果表明:65μmPDMS/DVB萃取头对较高沸点物质如苯甲醛、正辛醛、正辛醇、正壬醛、正壬醇的吸附比50/30μmDVB/CAR/PDMS萃取头要灵敏,但50/30μm DVB/CAR/PDMS萃取头对较低沸点物质的吸咐要灵敏很多。DVB/CAR/PDMS涂层萃取头所得的总峰面积的平均值最大,其余依次是PDMS/DVB、PA、PDMS;DVB/CAR/PDMS、PDMS/DVB、PA、PDMS对挥发物吸咐总峰面积与挥发物总量之间存在有线性关系,其相关系数(R2)分别是0.9939、0.9888、0.9176、0.9917;因此,65μm PDMS/DVB和50/30μm DVB/CAR/PDMS的萃取头较适用于茶油挥发物的提取。

Solid phase micro extraction （SPME） has been widely used for volatile extraction of gas, liquid and solid matrices. Four fibers, i.e. 100 μmPDMS, 85 μmPA, 65 μmPDMS/DVB and 50/30 μmDVB/CAR/PDMS, were selected for extraction in camellia oil volatile analysis. Results show that： （1） Benzaldehyde, octanal, octonol, nonanal, and nonanol which have relatively high boiling points are sensitive in 65 tan PDMS/DVB fiber, while the relatively low boiling point compounds are sensitive in 50/30 μm DVB/CAR/PDMS fiber; （2） The rank sequence of total peak areas in gas chromatography is DVB/CAR/PDMS 〉 PDMS/DVB 〉 PA 〉 PDMS; （3） Linear regressive relationship between the total peak area and volatile amount （0- 120 μg/g） in DVB/CAR/PDMS, PDMS/DVB, PA, PDMS exists with R2 of 0.9939, 0.9888, 0.9176 and 0.9917, respectively. These factors indicate that 65 μm PDMS/DVB and 50/30 tan DVB/CAR/ PDMS are suitable for the extraction of volatile of camellia oil.