中药思的明对吗啡依赖大鼠血清和尿中吗啡含量的影响

Effects of sidiming on serum and urine morphine concentration in morphine-dependent rats

目的：考察思的明（SDM）对吗啡依赖大鼠体内吗啡代谢的影响。方法：将大鼠随机分为吗啡依赖大鼠SDM治疗组（剂量以生药材计为8g·kg^-1·d^-1）、吗啡依赖大鼠非治疗组、吗啡依赖大鼠吗啡维持组、正常大鼠SDM对照组（剂量以生药材计为8g·kg^-1·d^-1）和正常大鼠生理氯化钠溶液（NS）对照组。前3组以剂量递增法连续皮下注射（s．c．）吗啡7d，建立吗啡依赖大鼠模型，d6开始各组分别给予相应的药物。后2组从d1开始分别灌胃（ig）给予SDM和NS。以上各组分别于d8～d11采集血样，d8～d13采集尿样。用乙酸乙酯提取血清及尿样中的吗啡，应用反相HPLC—UV法测定其浓度，并计算24h尿液中吗啡的排泄量。结果：与非治疗组比较，SDM治疗组吗啡依赖大鼠d8和d9血清吗啡含量明显增高，而d10和d11有一定程度降低；d8～d10吗啡排泄量明显增高，而d11～d13明显降低。吗啡维持组大鼠血清中吗啡含量均明显高于各组。NS对照组和SDM对照组大鼠血清中未检测到吗啡。结论：SDM能加快体内吗啡代谢和排出的速度，这可能是SDM有脱毒和促进机体康复功效的机制之一。

Objective：To study the effects of sidiming （SDM） on morphine metabolism in morphine-dependent rats in vivo. Methods： The rats were randomly divided into groups. A morphine-dependent model of rats was established by subcutaneous injection （s. c. ） of morphine at gradually increasing doses within 7 days. Oral SDM （8 g·kg^-1·d^-1） or saline （control） was administered from the 6th day （d 6） or the first day. The blood was collected from d 8 to d 11, and the urine Was collected from d 8 to d 13. Morphine was extracted from serum and urine by ethyl acetate, and determined by reverse phase HPLC-UV; the excreted quantity of morphine in the urine within the 24 h was calculated. Results： Compared with the group without SDM treatment, morphine content in the serum within d 8 and d 9 was significantly higher, but lower within d 10 and d 11 after SDM treatment in the morphine withdrawal rats. The excreted quantity of morphine in the urine was significantly higher within d 8 - d 10, but significantly lower within d 11 - d 13. Morphine content in the serum of morphine-treated group was significantly higher than those in other groups, but was not detected in both saline and SDM control groups without morphine treatment. Conclusion： SDM can accelerate the metabolism and excretion of morphine in the morphine-dependent rats, which may be one of the mechanisms related to detoxification and habilitation by SDM.