雪峰乌骨鸡自然群体遗传多样性的微卫星分析

Genetic diversity of the Xuefeng black bone chicken based on microsatellite markers

雪峰乌骨鸡是具有独特遗传特性的优质肉药兼用地方家禽品种。为了评价雪峰乌骨鸡现有品种资源的遗传背景、群体遗传结构等,提出合理的保种方法和措施,本研究筛选了家鸡基因组23条染色体上的23个微卫星标记,对采自湖南怀化的50只纯种乌骨鸡个体进行了多态性检测。结果表明:23个微卫星座位共检测到79个等位基因,所有座位都呈现出多态性,每个座位的等位基因数在2–6个之间,平均为3.435个。群体表现出较高的平均杂合度和平均多态信息含量,分别为0.6285和0.5496,说明雪峰乌骨鸡自然群体具有较丰富的遗传多样性。研究结果将为雪峰乌骨鸡种的保护和利用提供重要的理论依据。

We studied polymorphisms of 23 microsatellite loci from 23 chromosomes in an indigenous breed of Xuefeng black bone chicken from Hunan in order to elucidate its genetic diversity and structure and to propose reasonable measures for its conservation and utilization. Seventy-nine alleles were identified in 50 individuals and the mean number of alleles was 3.435 per locus. Average heterozygosity and polymorphic information content of 23 microsatellite loci were 0.6285 and 0.5496, respectively. These results indicate that this breed of Xuefeng black bone chicken has a high level of genetic diversity and may provide a scientific basis for future conservation and utilization of the Xuefeng black bone chicken.