VEGF—C及VEGFR-3在肝细胞癌中的表达

Expression of VEGF-C and VEGFR-3 in hepatocellular carcinoma and their significance

目的探讨VEGF—C、VEGFR-3、微血管密度（MVD）、微淋巴管密度（LVD）及肝细胞癌临床病理特征之间的关系。方法取肝细胞癌组织标本60例，正常肝组织标本20例。采用反转录聚合酶链反应（RT-PCR）方法分析其中VEGF—C及VEGFR-3mRNA的表达，以免疫组织化学法检测肝癌MVD及LVD，并分析四者与肝癌临床病理特点之间的关系。结果肝癌组织VEGF—C、VEGFR-3mRNA表达、MVD及LVD高于正常肝组织（P〈0．01）；肝癌组织中，VEGF—C与VEGFR-3表达、MVD及LVD均呈正相关（P〈0．01），VEGF—C及VEGFR-3表达与肝癌肝内转移、门静脉癌栓形成及淋巴转移相关（P〈0．01），MVD与肝癌肝内转移、门静脉癌栓形成相关（P〈0．01），LVD与淋巴转移相关（P〈0．01）。结论肝细胞癌组织中VEGF—C及VEGFR-3表达增多，可能通过参与血管、淋巴管生成促进肿瘤的侵袭、转移。

Objective To investigate vascular endothelial growth factor-c （VEGF-C） and vascular endothelial growth factor receptor-3（VEGFR-3） mRNA expression, microvessels density （MVD） and lymphatic mierovessels density （LVD） in human hepatocellular carcinoma and normal liver tissue. Try to illuminate the relationship among VEGF-C,VEGFR-3,MVD,LVD and the clinical pathological features of hepatoeellular carcinoma. Methods Liver tissue of 60 cases definitely diagnosed as hepatocellular carcinoma and 20 normal cases were collected. VEGF-C and VEGFR-3 mRNA expression were examined by RT-PCR, MVD and LVD were examined by immunohistoehemistry staining. Relationship between these indexes and clinical pathological features of hepatocellular carcinoma was also analysed. Results VEGF-C and VEGFR-3 mRNA expression, MVD and LVD in hepatocellular carcinoma were higher than those in normal liver tissue （P 〈 0.01）; In hepatocellular carcinoma tissue, expression of VEGF-C mRNA positively related with VEGFR- 3 mRNA, MVD and LVD（P 〈0.01）. VEGF-C and VEGFR-3 expression positively related with portal vein tumor thrombus, intrahepatal metastasis and lymph node metastasis （P 〈0.01）. MVD positively related with portal vein tumor thrombus and intrahepatal metastasis （P 〈0.01）. LVD positively related with lymph node metastasis （P 〈0.01）. Conclusion VEGF-C and VEGFR-3 expression increase in hepatocellular carcinoma tissue. They might play roles in tumor invasion and metastasis by inducing angiogenesis and lymphangiogenesis.