番茄红素对宫颈癌HeLa细胞bax、bcl-2基因表达的影响

Influence of lycopene on expression of bax and bcl-2 gene of HeLa cell

目的研究番茄红素对人宫颈癌HeLa细胞的诱导凋亡作用并探讨其对凋亡相关基因bax和bcl-2表达的影响。方法不同浓度番茄红素分别处理HeLa细胞24～72h后,MTT法检测细胞的生长活性,RT-PCR方法检测凋亡相关基因bax,bcl-2的表达。结果实验结果随着番茄红素浓度的增加,bax基因mRNA表达增加而bcl-2的mRNA表达减少。结论番茄红素对HeLa细胞的生长具有抑制作用,可促进宫颈癌HeLa细胞发生凋亡,bcl-2基因的减少与bax基因表达增加可能是HeLa细胞凋亡的机制之一。

Objective To study the influence of lycopene （Lyp） on apoptosis in HeLa cells and its relation with bax and bcl-2 gene expression. Methods The inhibitory effect of lycopene on HeLa cells was measured with MTT. The expressions of bax and bcl-2 gene were detected by RT-PCR. Results the expression of bcl-2 decreased but the expression of bax increased along with lycogen~ concentration. Conclusion lycopene can inhibit the growth of HeLa ceils, the down-regulation of bcl-2 and up-regulation of bax may be one of the mechanisms of the apoptosis.