黄花苜蓿双向电泳体系的建立

The Establishment of Two-Dimensional Electrophoresis Conditions for Proteome Analysis of Medicago falcata L.

通过对传统的双向电泳技术进行改进和优化，建立适合分析黄花苜蓿蛋白组学研究的双向电泳体系。结果表明，使用改进方法后所获得的双向电泳图谱稳定性和重复性较好，分辨率较高，经银染后可分辨出400多个蛋白点，其等电点主要分布于pI5～7，分子量主要分布于10～150kDa；经考马斯亮蓝染色后可分辨出800多个蛋白点，其等电点主要分布于pI5～7，分子量主要分布于10～150kDa。

Based on the conventional method of O＇Farrell, an improved procedure for the two--dimensional gel electrophoresis of Medicago falcata L. was presented. The two--dimensional separation of proteins was distinct and steady. The detected protein spots stained by silver nitrate were more than 400, with iso- electric points（pI） ranging mainly from 5 to 7 and molecular weights（MW） from 10 kDa to 150 kDa. Using Coomassie brilliant blue G-250, the detected protein spots stained were more than 800, with isoelectric points（pI） ranging mainly from 5 to 7 and molecular weights（MW） from 10 kDa to 150 kDa.