宿主细胞内SARS-CoV N蛋白相互作用蛋白的筛选与鉴定

Screen and Identification of The Protein-protein Interactors in The Host Cell With The SARS Coronavirus Nucleocapsid Protein

SARS-CoVN蛋白可与病毒RNA形成复合物,也可与病毒或宿主细胞中多种蛋白质相互作用,影响宿主细胞的多个信号转导通路,干扰宿主细胞的细胞周期,从而改变宿主细胞的生命活动.利用酵母双杂交技术筛选了15个宿主细胞内与SARS-CoVN蛋白的相互作用蛋白,其中包括信号传导组分7种,蛋白激酶3种,细胞因子2种,未知功能蛋白3种.并利用免疫共沉淀方法进一步证实了趋化因子CXCL16是宿主细胞与SARS-CoV核衣壳蛋白的相互作用蛋白.

15 SARS-CoV N Protein Interacting Protein （NPIP） were selected from host cells using Yeast Two-hybrid system （Y2H）. These are Angiogenin, acyglycerol kinase, cytochrome oxydase subunit I, CXC chemokine ligand 16, epidermal growth factor receptor pathway substrate 15, glutathione S-transferase kappa 1, integrin beta 1, jun oncogene, NIMA （never in mitosis gene a）-related kinase 10, protein tyrosine kinase 2 beta, homo sapiens SH3KBP1 binding protein 1 and ubiquitin specific peptidase 53. With the aid of immunological co-precipitation （CO-IP）, it was confirmed that chemokine CXCL16 was the interactor with SARS-CoV N protein in host cells.