亚麻耐盐性愈伤组织的生理生化特性

Physiological and Biochemical Characteristics of Salt-tolerance Callus of Flax

以亚麻（双亚5号）耐盐愈伤组织为材，以同种亚麻普通愈伤组织为对照，分别经含0～250mmol·L^-1 NaCl的培养基培养20d后，比较含水量、MDA含量、POD酶活、SOD酶活、EST同工酶谱、POD同工酶谱及SOD同工酶谱。结果表明：亚麻耐盐组愈伤组织与对照组愈伤组织在多方面存在明显差异，前者含水量均高于后者；丙二醛含量也高于后者，但变化幅度不大；POD酶活均高于对照组；酯酶同工酶谱不同于对照组，酯酶酶活量均高于对照组；POD同工酶谱比对照组多一条带，且酶活均高于对照组；SOD同工酶谱与对照组一样多，但酶活均高于对照组，且平稳。这为进一步筛选亚麻耐盐突变细胞系奠定了良好的基础。

The salt-tolerance callus of flax were used as experimental materials and common callus of flax were used as controls. After being cultured individually on the mediums containing 0 -250 mmol · L^-1 NaCl for 20 d, the water content, content of MDA, the POD activity, the SOD activity, esterase isozyme zymogram, POD isozyme zymogram and SOD isozyme zymogram were compared individually. The results indicated that there were obvious differences in many aspects between the callus of salt-tolerance groups and the callus of control groups of flax. The water contents, the contents of MDA and the POD activities in the former were all higher than those in the latter as well as esterase activities. Esterase isozyme zymograms of callus of salt-tolerance groups were different from those of callus of contrast groups. There was more POD isozyme band in salt-tolerance groups. SOD isozyme bands in salt-tolerance groups were as many as those in control groups, SOD quality in the former was higher and stable. The stody laid a sound base for the further screening of salt-tolerance mutant cell line of flax.