摘要
目的探讨SLE患者中干扰素调节因子-1(IRF-1)基因的表达情况及泌乳素(PRL)对其干预作用。方法电化学发光仪检测30例SLE患者与20例正常人对照组在清晨静息状态下的血清PRL水平,按密度梯度离心法分离、培养外周血PBMC,将样本分为SLE患者组、正常人对照组、正常PRL的SLE患者组+重组人泌乳素(rhPRL)和正常人对照组+rhPRL等4组。应用RT—PCR结合凝胶图象扫描技术检测IRF-1基因的表达情况:结果SLE患者组与正常人对照组IRF-1基因表达的相对值分别为0.89±0.21和0.78±0.18,两组比较,差异有统计学意义(P=0.026),高PRL的SLE患者组为1.06±0.26,显著高于正常PRL的SLE患者组的0.82±0.21(P=0.005),而正常PRL的SLE患者组与正常人对照组间差异无统计学意义(P=0.514)。经rhPRL刺激后,正常PRL的SLE患者IRF-1基因表达的相对值为0.99±0.22,显著高于rhPRL刺激前(P=0.036),而正常人对照组在刺激前后差异无统计学意义。结论SLE中存在IRF-1基因的表达异常,高PRL水平可能是导致IRF-1基因异常表达的原因之一。
Objective To study the expression of interferon regulatory factor 1 (IRF-1) in patients with systemic lupus erythematosus (SLE) and the influence of prolactin (PRL) upon it. Methods The level of serum PRL in quiescent condition was examined by electrochemiluminescence-meter in 30 patients with SLE and 20 healthy controls. Peripheral blood mononuclear ceils i PBMC) were separated from all the subjects by gradient centrifugation density, and cultured PRL (rhPRL) for 24 hours. The expression of IRF-1 with or without the presence of recombinant human gene in cultured PBMC was detected by reverse transcriptase-PCR (RT-PCR) with gel image scanning. Results The relative value of IRF-1 gene expression was significantly higher in SLE patients than in normal controls (0.89 ± 0.21 vs 0.78 ± 0.18, P = 0.026), and in SLE patients with high PRL than in those with normal PRL (1.06 ± 0.26 vs 0.82 ± 0.21, P=0.005). However, there was no significant difference between SLE patients with normal PRL and healthy controls in regard to the expression of IRF-1 gene (P = 0.514). The stimulation with rhRPL significantly elevated the relative expression of IRF-1 gene in SLE patients with normal PRL (0.99 ± 0.22 vs 0.82 ± 0.21, P = 0.036), but had no obvious effect on that in the normal controls. Conclusion The study reveals a high expression of IRF-1 gene in SLE patients, which may be related to the high level of PRL.
出处
《中华皮肤科杂志》
CAS
CSCD
北大核心
2008年第9期579-581,共3页
Chinese Journal of Dermatology
基金
广东省医学科研课题(A2000379)
关键词
红斑狼疮
系统性
催乳素
干扰素调节因子-1
Lupus erythematosus, systemic
Prolactin
Interferon regulatory factor 1