TgN(p53mt-LMP1)/HT小鼠鼻腔和鼻咽黏膜上皮组织生物学表型特征及细胞周期特点分析

Biological phenotype and cell cycle distribution characteristics of nasal and/or nasopharyngeal epithelia in TgN(p53mt-LMP1)/HT mice

目的:研究TgN(p53mt-LMP1)/HT小鼠鼻腔和鼻咽黏膜上皮组织生物学表型特征及细胞周期分布特点。方法:H-E染色法观察正常饲养的TgN(p53mt-LMP1)/HTG4代5、11、15和18月龄阳性表达和阴性表达小鼠鼻腔和鼻咽黏膜上皮组织生物学表型特征(主要是癌前病变率),流式细胞术测定其细胞周期分布特点。结果:5、11、15和18月龄阳性表达小鼠鼻腔和鼻咽黏膜上皮组织的癌前病变率分别为0、50%、60%和75%,不同月龄阴性表达小鼠的癌前病变率均为0。与转基因阴性小鼠比较,转基因阳性小鼠鼻腔和鼻咽黏膜上皮组织G0/G1期细胞数量减少,S期、G2/M期细胞数量增多,细胞增殖指数(PI)增高(P<0.01)。结论:随着年龄增长,TgN(p53mt-LMP1)/HT小鼠鼻腔/鼻咽黏膜上皮组织癌前病变率增加,PI增高,表明鼻腔/鼻咽癌前病变转基因小鼠模型构建成功。

To investigate the biological phenotype and cell cycle distribution characteristics of nasal and/or nasopharyngeal epithelia of TgN （p53mt-LMP1）/HT mice. Methods： The biological phenotype changes （ mainly the incidence of precancerous lesion） in nasal and nasopharyngeal epithelial tissues of the generation G4 of TgN（p53mt-LMP1 ）/HT mice aged 5, 11, 15 and 18 months were deter- mined by H-E staining, and the cell cycle characteristics of nasal and nasopharyngeal epithelia detected by flow cytometry （FCM）. Results ： The incidences of precancerous lesion in the nasal and nasopharyngeal epithelia were 0, 50% , 60% and 75% respectively in the 5, 11, 15 and 18 mos groups of the positively expressed transgenic mice, and 0 in the four age groups of the negatively expressed ones. Compared with the negatively expressed transgenic mice, the number of nasal or nasopharyngeal epithelial cells was markedly decreased in the G0/G1 phase, but obviously increased in the S and G2/M phases, with the proliferation index （PI） significantly enhanced （P 〈 0.01 ）. Conclusion： The incidence of precancerous lesion in the nasal and/or nasopharyngeal epithelia of TgN（ p53mt-LMP1 ）/HT mice rises with the age,the cellular proliferating potentiality strengthened and the PI increased. The transgenic mouse model of precancerous lesion in nasal and/or nasopharyngeal epithelia have been constructed successfully.