重组腺病毒载体介导报告基因经多种途径转染至大鼠肾脏

Recombinant adenovirus vector-delivered report gene expression in rats kidney through different ways

目的研究重组腺病毒(rAV)介导报告基因绿色荧光蛋白(GFP)能否转染到大鼠肾组织,以及多种途径转染的差异。方法30只大鼠随机分成5组,经5种途径注射rAV-GFP(1013个病毒颗粒·L-1)溶液,注射后2、7和14d,分别取各组大鼠的左肾、右肾、肝脏和肺,荧光显微镜观察,并通过计算机软件评价报告基因在肾组织中的表达情况。结果各种注射方式均可使绿色荧光蛋白在肾中表达,注射后7d时GFP表达达到高峰,并以腹腔和尾静脉注射方式表达最强;经肾动脉注射的靶向性较其他方式好。结论rAV载体能携带报告基因到肾组织,rAV可以用于肾疾病的基因治疗,且各种注射方式各有优缺点。

Objective To investigate expression of recombinant adenovirus （rAV） vector-mediated report gene in kidney, and to seek for the best passway of rAV vector-mediated gene transfer in vivo. Methods Thirty Sprague- Dawley（SD） rats were divided into 5 groups randomly, rAV-GFP（ 1013viral particales · L^-1） was injected through different ways. 2, 7 and 14 d after injection, GFP expression in left kidney, right kidney, liver and lung was eval- uated by fluorescence microscope and computer software. Results GFP gene expressed in kidey and reached peak 7 d after injection expression. Conclusion rAV vector-mediated report gene can be transducted in kidney. The rAV-mediated transgene expression in kidney is a potential strategy in the treatment of renal diseases.