TLC-可见分光光度法对丝瓜藤及其炮制品质量控制的应用

Application of TLC-Visible Spectrometry Method in Quality Control of Stem of Suakwa Vegetablesponge (Luffa cylindrica) and its Processed Product

用薄层刮板-可见分光光度法检测丝瓜藤及其炒黄、炒炭制品中齐墩果酸的含量。其方法回收率为99．52％，r=0．9998（n=5），RSD为1．05％，取得了较为可靠的结果。证明丝瓜藤原药材及其炮制品中齐墩果酸含量无显著差别。

A TLC-visible spectrometry method for the determination of oleanolic acid in stem of Luffa cylindrica (L.)Roem and its processed product (stir baked to brown or charred)was reported. The method was carried out on thin layer plates of silica gel G with chloroform methyl-alcohol (10: 1)as the developer,and visible absorbance of the eluate was determined at 450 nm. The method showed good linearity(r=0.9998,n= 5)average recovery was 99. 52%,and RSD was 1.05 %. Results showed that the Oleanolic acid content in the stem of L. cylindrica and its processed product was not distinctly different from each other.