血浆中萘替芬的HPLC测定

HPLC DETERMINATION OF NAFTIFINE IN PLASMA

采用ＨＰＬＣ法测定家兔血浆中萘替芬的浓度，线性范围０．１～５．０μｇ／ｍｌ，检测极限３０ｎｇ／ｍｌ，日内、日间误差＜５％。药动学研究表示，本品药动学符合二室模型（家兔静注）。

A HPLC determination of naftifine in plasma has been developed. Mobile phase was methanol acetonitrile 0.025 mol/L NH 4OAc H 3PO 4 buffer (70∶5∶25, pH 6.0). Cyproheptadine was chosen as internal standard. Detection limit in plasma was 30 ng/ml. The RSD s within-and between-day ≤5.0%. The iv results in rabbits showed that naftifine was eliminated following first order kinetics and two compartment model: C= 5.18 -13.72 t +0.69 0.09 t .