根系分泌物体系中五氯酚测定方法研究

Determination of Pentachlorophenol in Root Exudates of Sunflower by Ultraviolet Spectrophotometric Method

以向日葵（Helianthus annnus）的根系分泌物为研究对象，建立了采用紫外分光光度法测定根系分泌物中疏水性有机污染物PCP方法。实验结果如下：最佳吸收波长为215nm；标准曲线方程为Y=0．1269X＋0．1193，r=0．9997；方法检出限为0．025mg／L；样品加标回收率为61．01％～104．98％，相对标准偏差低于0．3％。可见，利用此方法测定根系分泌物体系中PCP具有灵敏度高、准确度好、操作简单、快捷，重复性好等优点，适宜于研究植物修复过程中五氯酚的批量测定。

Taking the root exudates of sunflower as study object, the determination of the hydrophobic organic pollutants PCP in root exudates was set up by UV spectrophotometer. Results as follows ：the best determing wavelength was 215 nm, the linear regressive equation was Y = 0. 126 9X ＋0. 119 3, the correlation coefficient r was 0. 999 7 ; the detection limit were 0. 025 mg/L; the recovery range was 78.48% - 103.93% ,the relative standard division （RSD） obtained was less than 3% （n = 6）. This method is convenient, fast and good reproducibility, sensitivity and accuracy, it＇ s applicable for bath determination of pentachlorophenol in the course of study its phytoremediation.