子宫颈鳞癌中MHC-Ⅰ类呈递抗原相关蛋白表达与HPV16相关性的研究

Association of expression of MHC class Ⅰ antigen presentation associated proteins with HPV16 in cervical cancer

目的研究子宫颈炎组织和子宫颈鳞癌组织中MHC-Ⅰ类抗原呈递相关蛋白(TAP1、TAP2、β2-MG、HSP70)在维吾尔族和汉族妇女中的表达差异。方法应用免疫组化SP法和聚合酶链反应技术检测子宫颈炎组织以及子宫颈鳞癌组织中HPV抗原呈递相关蛋白TAP1、TAP2、β2-MG和HSP70的表达和HPV16的感染情况。结果TAP1、TAP2和β2-MG在子宫颈鳞癌组织中阳性表达率(51%、60%和61%),均低于子宫颈炎组织(77%、95%和96%,P均<0.05)。在子宫颈鳞癌组织中,维、汉族间TAP1、TAP2和β2-MG阳性表达有差异(P<0.05)。在子宫颈炎组织中,维、汉族间TAP1、β2-MG阳性表达有差异(P<0.05)。维吾尔族子宫颈鳞癌中β2-MG表达与HPV16感染关系为正相关(r=0.216,P<0.05)。汉族子宫颈鳞癌中,TAP2与β2-MG间存在正相关(P<0.05)。维吾尔族子宫颈鳞癌中,TAP1与β2-MG、TAP1与HSP70间存在正相关(P<0.05)。结论子宫颈鳞癌中TAP1、TAP2和β2-MG的表达量下降,导致细胞表面MHC-Ⅰ类分子表达降低,可能是肿瘤细胞逃逸免疫监视的一种机制,且在维吾尔族和汉族间相关性呈现差异,提示不同蛋白在不同民族间子宫颈鳞癌的发病机制方面可能存在不一致。

Purpose To study the expression of MHC class I antigen presentation associated proteins TAP1 , TAP2 , HSP70 and β2- MG in cervical cancer lesions and its correlation with HPV16 infection in cervical cancer patients of Uighur and Han women. Methods Immunohistochemical S-P method and polymerase chain reaction （PCR） were applied for detection of expression of MHC antigen presentation associated proteins TAP,, TAP2 , β2-MG and HSP70, and infection of HPV16, respectively, in cervicitis tissues and cer- vical cancer lesions from Han and Uighur women. Results The expression rates of TAP1 , TAP2 and β2-MG in cervical cancer tissues from both Han and Uighur women （51%, 60% , 61% , respectively） were significantly reduced when compared to mose in cervicitis tissues from both ethnic groups （77%, 95%, 96%, respectively, P 〈 0. 05）. The difference in positive expression rate of TAP1, TAP2 and β2-MG in cervical cancer tissues were significant between Han and Uighur women （ P 〈 0. 05 ）, whereas the positive expression rate of TAP1 and β2-MG between Uighur and Han women was significantly different （ P 〈 0.05 ）. The expression rate of β2-MG was positively correlated with infection of HPV16 in cervical cancer lesions from Uighur woman （ r = 0. 216, P 〈 0. 05）. There was positive correlation in expression of β2-MG and TAP2 in cervical cancers of Han women （ P 〈 0. 05 ） , and there were positive correlations between expression of β2-MG and TAPl , and TAP1 and HSP70 in cervical cancer lesions from Uighur women. Conclusions Decrease in the expression of TAP1 , TAP2 and β2-MG in cervical cancer lesions leads to the down-regulation of MHC class I molecules in the cancer cells and reduced antigen presentation efficiency, which may be the one of the mechanisms for cancer cells to escape immune surveillance. The different association of expression of these proteins with HPV16 infection between Han and Uighur women suggests possible discrepancies of these proteins in the carcinogenesis in different ethnic groups.