胃癌中iNOS与HIF-1α表达的相互关系及临床意义

Relationship between hypoxia-inducible factor-1α and inducible nitric oxide synthase expression in gastric cancer and its clinical significance

目的:研究诱导型一氧化氮合酶(iNOS)与缺氧诱导因子-1α(HIF-1α)在胃癌中表达的相互关系,探讨他们在胃癌的发生发展、浸润和转移中的作用.方法:2001-01/2002-03手术切除,病理证实为腺癌的胃癌标本54例,患者术前均未行化疗或放疗,另取正常胃组织15例作对照.应用免疫组化SP法检测胃癌及正常胃组织中iNOS和HIF-1α的表达,光镜下观察iNOS及HIF-1α蛋白表达,分析iNOS与HIF-1α的表达关系及临床意义.结果:胃癌中iNOS和HIF-1α的表达明显高于正常胃组织(66.67%vs6.67%P<0.05;74.07%vs0%P<0.01),其表达与胃癌TNM分期、肿瘤浸润深度和淋巴结转移显著相关(P<0.05或0.01).胃癌组织中iNOS与HIF-1α的表达呈正相关(r=0.596,P<0.05).结论:iNOS和HIF-1α在胃癌的发生发展中起重要作用,联合检测可作为判断胃癌恶性程度和预后的指标.

AIM： To investigate the expression of inducible nitric oxide synthase （iNOS） and hypoxia-inducible factor-1α （HIF-1α） as well as their correlation in gastric cancer, and to explore their roles in the genesis, invasion and metastasis of gastric cancer. METHODS： Immunohistochemical technique was used to detect the expression of iNOS and HIF-1α in 54 cases of gastric cancer and 15 cases of normal gastric tissues. The relationships between the expression of iNOS, HIF-1α and tumor angiogenesis, clinical pathologic features of patients were analyzed. RESULTS： The expression levels of iNOS and HIF-1α in gastric cancer were significantly higher than those in the normal gastric tissues （66.67% vs 6.67%, P 〈 0.05; 74.07% vs 0%, P 〈 0.01）; iNOS and HIF-1α expression in gastric cancer were significantly associated with TNM staging, invasive depth and lymph node metastasis （P 〈 0.05 or 0.01）. There was a positive correlation between iNOS and HIF-1α expression （r = 0. 596, P 〈 0.05）. CONCLUSION： Inducible NOS and HIF-1α play very important roles in the genesis and development of gastric cancer, and they may serve as markers in the diagnosis of gastric cancer and estimation of prognosis.