摘要
目的:为研制蝰蛇毒压电免疫传感器,研究抗蛇毒抗体固定于石英晶体银电极表面的固定技术。方法:采用马抗蝰蛇毒血清抗体和抗蝰蛇毒鸡卵黄抗体作为生物敏感材料,对比研究了胱胺自组装-PSS反相吸附法和PEI粘附-戊二醛交联法;比较了采用两种固定方法所制的压电免疫传感器的性能。结果:鸡卵黄抗体采用PEI粘附-戊二醛交联法效果较好,其制备的IgY压电免疫传感器检测蝰蛇毒灵敏度为0.5μg/mL;而马血清抗体用胱胺自组装-PSS反相吸附法较好,其制备的IgG’免疫传感器检测蝰蛇毒灵敏度为10μg/mL。结论:以PEI粘附-戊二醛交联法固定抗蝰蛇毒鸡卵黄抗体所制备的蝰蛇毒压电免疫传感器的性能稳定,特异性好,可实现蛇毒的快速检测。
Objective： To develop a piezoelectric imrnunosensor for Viper venom detection, two kinds of immobilization methods were studied. Methods： Anti-Viper venom egg yolk antibody IgY and refined horse serum antibody against Viper venom were fixed on silver electrode surface of the quartz crystal to make piezoelectric immunosensors by PEI-GA cross-linking method and CYS-PSS SAMs method. Results： PEI-GA cross-linking method, was preferable for IgY immobilization, and the sensitivity of the immunosensor by this method was 0.5 ug/mL. CYS-PSS SAMs method, was preferable for refined horse serum antibody immobilization, and the sensitivity of the immunosensor was 10 ug/mL. Conclusions： a piezoelectric immunosensor was made from anti-Viper venom IgY by PEI-GA cross-linking immobilization method. It has good stability and specfictive in fast detection for snake venom.
出处
《中国医学物理学杂志》
CSCD
2008年第5期810-813,822,共5页
Chinese Journal of Medical Physics
基金
广州市科技局:穗科条字[2005]6号
广东省自然科学基金(No.204102537)
