高效液相色谱法测定兔血浆中黄芩苷的含量

Determination of Baicalin Concentration in Rabbit Plasma by RP-HPLC

目的：建立测定兔血浆中黄芩苷的含量测定方法。方法：用PEG400—2％偏磷酸沉淀血浆蛋白，离心后取上清液进行色谱分析。色谱柱为Diamosil—C18，流动相为乙腈-0．1％磷酸（25：75，V／V），流速为1ml／min，检测波长275nm。结果：黄芩苷的质量浓度在0．08722～2．9075mg/L（r=0．9899）；低、中、高3个浓度的质量控制（QC）样品的相对回收率分别为96．3％、97．3％和96．6％。结论：方法简便快速，适用于黄芩苷的血药浓度测定及药代动力学研究。

Objective： To establish a biopharmaceutical analytical method for the determination of baicalin in plasma by RP-HPLC. Methods： Rabbit plasma proteins were precipitated with PEG400- 2% metaphosphoric acid and the supernatant liquid obtained from the serum by centrifuge was subjected to chromatographic analysis. The analytical column was Eclipse XDB-C18, and the mobile phase consisted of acetonitrile-0.1% phosphoric acid （25： 75, V/V） with a flow rate of lmL/min. The detection wavelength was 275 nm. Results：The linear range of baicalin in rabbit plasma was 0. 087 22 2.907 5 mg/L （ r = 0.989 9）. Three relative recoveries of baicalin represented low, middle and high concentrations were 96.3% ,97.3% and 96.6% respectively. Conclusion： This method is accurate, reliable and simple and is suitable for the pharmacokinetic study of blood concentration of baicalin.