血管紧张素转换酶基因多态性和酗酒与缺血性脑卒中的关联

Associations of Ischemic Stroke and Angiotensin Converting Enzyme Gene Polymorphism and Alcohol Abuse

目的探讨血管紧张素转换酶(ACE)基因多态性和酗酒与缺血性脑卒中(IS)发病风险的关系。方法应用聚合酶链反应(PCR)和变性高效液相色谱(DHPLC)技术筛查了454例IS患者(病例组)和334例非IS患者(对照组)的ACE基因的多态分布,采用非条件Logistic回归模型分析基因型、酗酒情况与缺血性脑卒中患病的关系。结果与对照组相比,酗酒群体的DD基因型和D等位基因的频率显著性升高(P<0.05),患缺血性脑卒中的相对危险度分别为8.130和2.488;而携带有Ⅱ基因型的酗酒群体患缺血性脑卒中的相对危险度则为0.389。相反,非酗酒IS患者的ACE基因的各基因型和等位基因频率的分布与对照组相比,均无显著性差异(P>0.10)。结论携带有D等位基因的酗酒群体容易患缺血性脑卒中,但携带有Ⅰ等位基因的酗酒群体不容易患缺血性脑卒中,ACE基因与酗酒在缺血性脑卒中的发病过程中存在协同作用。

Objective To study the associations of ischemic stroke （IS） and angiotensin converting enzyme （ACE） gene polymorphism and alcohol abuse. Methods 454 patients and 334 controls were recruited in our study. The polymorphism of ACE gene was detected by polymerase chain reaction （PCR） and denaturing high performance liquid chromatogram （DHPLC）. The relationships between the genotypes and alcohol abuse and ischemic stroke risk were measured by odds ratios （ORs） and 95% confidence intervals （95% CIs）. The ORs and 95% CIs were calculated by unconditional logistic regression. Results Compared to controls, the frequencies of DD genotype and D alleles in ischemic stroke patients with alcohol abuse were significantly higher than those in controls, with the OR of 8.130 and 2.488; respectively. The OR of genotype Ⅱ of ischemic stroke patients with alcohol abuse was 0.389. On the other hand, the OR of all genotypes and alleles in ischemic stroke patients with non-alcohol abuse were not significantly different from those in controls. Conclusion They indicated that those alcohol abusers with D alleles are likely to suffer from ischemic stroke, but those alcohol abusers with I alleles are not likely to suffer from ischemic stroke. There is likely a correlation between alcohol abuse and ACE gene polymorphism in the pathogenesis of ischemic stroke.