四种梅毒血清学实验检测方法的比较

The Comparative Study of Four Serologic Tests for Syphilis

目的比较四种梅毒血清学实验检测方法的准确性及在临床诊断中的价值。方法分别用甲苯氨红不加热实验(TRUST)、酶联免疫吸附法(ELISA)、梅毒螺旋体明胶颗粒凝胶实验(TPPA)、化学发光法检测180例梅毒血清样本。结果TRUST、ELISA、TPPA、化学发光法的敏感性分别为86.11%、97.78%、99.44%、98.89%,特异性分别为81.25%、98.75%、100%、98.75%。以TPPA作为标准,ELISA和化学发光法的敏感性高于TRUST,差异有统计学意义(P<0.05),特异性ELISA和化学发光法间差异无统计学意义(P>0.05)。结论ELISA和化学发光法可作为TPPA的替代实验,在临床中广泛应用。

Objective To compare and evaluate the result of four serologic tests to syphilis serum specimens of different stages. Methods Four approaches including TRUST, ELISA, TPPA and Chemiluminescent Immunoassays were used to detect syphilis in 180 serum specimens. Results The sensitivities of the four approaches （TRUST, ELISA, TPPA and Chemilumineseent Immunoassays） were 86.11%,97.78%,99.44%,and 98.89%, respectively, while the specificities were 81.25%, 98.75%, 100%, and 98.75%, respectively. TPPA was used as the gold standard. The sensitivities of the ELISA and Chemiluminescent hnmunoassays were higher than that of the TRUST（P〈0.05）. The specificities of the ELISA and Chemiluminescent Immunoassays were similar. Conclusion The ELISA and Chemiluminescent Immunoassays were better methods to detect syphilis specimens of different stages.