含腺嘌呤的杯芳烃衍生物的合成、表征及其对核苷碱基的分子识别性质

Synthesis and Characterization of p-tert-Butylcalix[4]arene Derivative Containing Adenine and Its Molecular Recognition to Nucleotides and Bases

设计合成了一种新型含腺嘌呤基的杯[4]芳烃衍生物(AC)——5,11,17,23-四叔丁基-25,27-二羟基-26-[1-(9-腺嘌呤)-丙氧基]-28-溴丙氧基杯[4]芳烃,通过红外光谱、元素分析、核磁共振谱和电喷雾质谱等手段对其进行了表征,确认为目标产物;并采用紫外分光光度法研究了AC的分子识别作用,通过测定AC与不同浓度、不同组分的核苷、碱基混合体系的吸光度,证明了AC对尿嘧啶、尿苷具有分子识别作用,能从其它核苷或碱基共存的体系中将尿苷或尿嘧啶识别出来.

A new adenine-containing derivative of p-tert-butylcalix [ 4 ] arene （ AC ） -- 5,11,17,23-tetra-t- butyl-25,27-bihydroxy-26- （ 1 - （ 9-adenineyl ） -propoxy ） -28-bromopropoxycalix [ 4 ] arene was synthesized and characterized via IR, EA, 1H NMR and ESI-MS spectra. The molecular recognition of AC to different nucleo- tides and bases was studied via UV-Vis method ： the absorbance of mixed systems with different concentrations or components was measured. The results show that AC can selectively bond with uracil and uridine from other nucleotides and bases, which means AC can recognize uracil and uridine.