真菌诱导子对象牙白原球茎生长的影响

Effects of fungal elicitors on the protocorm of Cymbidium eburneum

在1/2MS基本培养基中,分别加入由野生春兰和华石斛根部分离到的内生真菌所制成的4种菌丝提取物,对象牙白种子萌发形成的原球茎进行诱导培养。结果表明:4种菌株诱导子中3种可不同程度地促进象牙白的生长和分化。对象牙白原球茎的诱导作用依次为HF26>CF6>CF8,与对照相比,HF26菌株显著提高了象牙白的鲜重(P<0.05),鲜重增长率分别为96.13%、86.86%和61.18%;干物质重分别增加了130.70%、96.50%和124.30%,均达到极显著水平(P<0.01)。此外,CF8菌株能促进根条数的增加;HF26菌株最有利于原球茎的增殖;CF6菌株在促进成苗和根原基的分化上效果显著。结论:在象牙白原球茎生长的不同阶段,能够促进其达到最佳生长状态的菌株是不同的。

Growth of protocorm of Cymbidium eburneum induced on basal culture medium of 1/2 MS by four fungal elicitors,which were isolated from the roots of Cymbidium goeringii and Dendrobium sinens was examined in our study.The results showed that three fungal elicitors out of four considerably promoted the growth and differentiation of protocorm,and that the inducing effects were in the order of HF26〉CF6〉CF8.Compared with the control group,HF26 strain was found to increase the fresh weight of C.eburneum（P〈0.05）.The increased rates in fresh weight were 96.13%,86.86% and 61.18%,while the increased rates in dry weight were 130.70%,124.30% and 96.50%,respectively（P〈0.01）.In addition,CF8 strain could promote the number of roots;HF26 strain was beneficial to multiplication of protocorm;CF6 strain had a remarkable effect on seedling establishment and differentiation of root primordia.It can be concluded that different fungus have different effects on the growth of protocorm of Cymbidium eburneum in different growth stages.