清热利湿中药对梗阻性黄疸肝细胞凋亡及Bcl-2表达的影响

Effect of Heat-clearing and Dampness-removing Herbs on Hepatic Cell Apoptosis and BCL-2 Expression in Rats with Obstructive Jaundice

目的:探讨清热利湿中药对梗阻性黄疸大鼠肝细胞凋亡及Bcl-2表达的影响。方法:建立梗阻性黄疸大鼠模型,在此基础上将模型大鼠分为中药组和盐水组、模型组,并设立假手术组作对照。分别于第3、7、14、21天处死大鼠,观察血清胆红素、肝组织细胞凋亡指数(AI)、Bcl-2表达等。结果:模型组血清胆红素水平明显升高,与假手术组比较,差异有显著性意义(P<0.05),提示造模成功。盐水组与中药组分别在第3、7、14、21天各时间段血清胆红素比较,差异均无显著性意义(P>0.05).提示清热利湿中药不能从根本上降低血清胆红素;随着时间推移,血清胆红素将逐渐增高。不同时期盐水组、中药组AI均较高,与假手术组比较,差异均有显著性意义(P<0.05),但中药组不同时期AI均较盐水组低(P<0.05)。假手术组Bcl-2表达较低,与盐水组第3、7天比较,差异无显著性意义(P>0.05),但与盐水组第14天、第21天比较,差异有显著性意义(P<0.05);假手术组与中药组第3、7天比较,差异无显著性意义(P>0.05),但与中药第14、21天比较,差异有显著性意义(P<0.05)。盐水组第3、7天与中药组同时期比较,差异无显著性意义(P>0.05),盐水组第14、21天与中药组同时期比较,差异有显著性意义(P<0.05)。结论:清热利湿中药可促进梗阻性黄疸状态下Bcl-2表达,抑制肝细胞凋亡。

Objective:To observe the effect of heat-clearing and dampness- removing herbs on hepatic cell apoptosis and BCL-2 expression in rats with obstruc- tive jaundice.Methods:Rat models of obstructive jaundice were established,and then were divided into herbs treatment group and normal saline group.The rats were executed on the 3rd,7th,14th and 21st day respectively,and the serum bilirobin content,hepatic cell apoptosis index(AI)and Bc1-2 expression were observed. Results:Serum bilirubin content was increased in the model group,and the differ- ence was significant as compared with that in the pseudo-operation group(P<0.05),indicating the success of the modeling.The difference of serum hilirubin con- tent in herbs treatment group and normal saline group on the 3rd,7th,14th and 21st day was insignificant(P>0.05),indicating that the herbs could not thoroughly clear the serum hilirubin.With the prolongation of modeling time,the serum bilirobin con- tent was increased.AI in herbs treatment group and normal saline group at different time was increased(P<0.05 compared with that in the pseudo-operation group), and AI in herbs treatment group was lower than that in normal saline group(P<0.05).Bcl-2 expression in the pseudo-operation group did not differ from that in the normal saline group on the 3rd and 7th day(P>0.05),but differed from that in the normal saline group on the 14th and 21st day(P<0.05).Bcl-2 expression in the pseudo-operation group did not differ from that in herbs treatment group on the 3rd and 7th day(P>0.05),but differed from that in herbs treatment group on the 14th and 21st day(P<0.05).The difference of Bcl-2 expression in the normal saline group was insignificant on the 3rd and 7th day(P>0.05),but was significant on 14th and 21 st day(P<0.05).Conclusion:Heat-clearing and dampness-re- moving herbs can inhibit hepatic cell apoptosis and promote BCL-2 expression in rats with obstructive jaundice.