AngⅡ受体阻断剂对系膜增殖型肾炎家兔系膜基质的影响

Effect of lonsartan on mesangial matrix of mesangial proliferative glomerulonephritis rabbits

目的探讨血管紧张素Ⅱ受体阻断剂洛沙坦对系膜增殖型肾炎家兔系膜细胞增殖、系膜基质增多的的药效和作用特点。方法将雄性日本大耳白兔30只随机分成正常对照组、病理模型对照组和洛沙坦治疗组。建立家兔系膜增殖型肾炎模型。双缩脲法测定洛沙坦对系膜增殖型肾炎家兔24h尿蛋白排泄量;放免法测定血浆中内皮素及降钙基因相关肽的水平;自动图像分析仪测定各组肾小球的定量指数,系膜基质指数;用免疫组化法观察肾组织增殖性细胞核抗原(PCNA)表达。结果与病理模型对照组相比,洛沙坦治疗组家兔24h尿蛋白排泄量明显下降(P<0.05),系膜面积显著减少(P<0.05),内皮素(ET-1)降低、降钙素基因相关肽(CGRP)升高(P<0.05),PCNA阳性细胞数减少(P<0.05)。结论洛沙坦具有调节内皮素与降钙素基因相关肽病理性改变,减少系膜基质的增多,及系膜细胞的增殖,从而对肾炎家兔有明显的保护作用。

Objective To investigate the effect of angiotensin Ⅱreceptor antagonist, lonsartan on the clinic histological change of MSPGN rabbits. Methods 30 male rabbits were randomly divided into 3 groups： The normal control groups（ N groups）. The untreated groups（ M group）. The lonsartan treated groups（ L group）. The rabbits mesangial proliferative glomerulonephritis （ MSPGN ） models were established. 24 hours excretion quantity of urine protein（EQUP） was determined every week ,the levels of plasma endothelin-1 （ET-1）and Calcitonin gene-related peptide （CGRP）were tested with radioimmunoassay（RIA） mesangial Matrix expansion were evaluated with quantitative pathologic analysis, immunohistochemical stain for proliferating cell nuclear antigen （PCNA）was performed after treatment. Results EQUR mesangial matrix expansion ET-1 in L group were significantly lower than those in M group（ P 〈 0. 05 ） While CGRP was remarkably higher（ P 〈 0.05 ）. The number of PCNA was less remarkably（P 〈 0. 05 ）. Damage of renal tissue in L group was slighter compared with that in M group. Conclusion Captopril can decrease mesangial matrix expansion, alleviate the injury of renal tissue and obtain markedly therapeutic effect.