化学发光法测定枳壳中生物碱含量

Determination of Alkaloids in Fructus Aurantii by Chemiluminescence Analysis

在酸性介质中,高锰酸钾可氧化辛弗林产生化学发光,对影响化学发光强度的因素进行了实验和探讨,建立了高锰酸钾化学发光体系测定辛弗林的新方法。在最佳实验条件下,辛弗林的线性范围为4.00×10-7—2.00×10-5mol/L;检出限为6.96×10-8mol/L。该体系已经成功应用于枳壳中生物碱含量的测定,回收率为92.8%。此外,本文还测定了酸性高锰酸钾-辛弗林体系的化学发光光谱和荧光光谱,初步探讨了化学发光反应机理。

A new method was established for the determination of alkaloids in fructus aurantii based on acidic potassium permanganate chemiluminescence （CL）. The optimum conditions for the chemiluminescence reaction between synephrine and potassium permanganate were studied in detail. Under the optimum conditions,the linear range for synephrine is 4.00 × 10^-7-2. 00 × 10^-5mol/L with the detection limit of 6. 96 × 10^-8mol/L. This method was applied to determine the content of alkaloids in fructus aurantii and the recovery is 92. 8 %. In addition,a possible mechanism of this CL reaction was proposed on the basis of the investigation of CL and fluorescent spectra of synephrine in acidic solution containing potassium permanganate.