嗜酸乳杆菌细胞壁肽聚糖的分离提取

Extraction of Peptidoglycan in Lactobacillus acidophilus Cell Wall

分别采用超声波法、溶菌酶法、三氯乙酸法对嗜酸乳杆菌细胞壁肽聚糖进行分离提取。结果表明超声波法的最优组合为超声功率400W,超声时间30min,水浴温度40℃,肽聚糖得率10.2%;溶菌酶法的最优组合为溶菌酶添加量4mg/mL,水浴温度55℃,作用时间5h,pH7,肽聚糖得率10.0%;三氯乙酸法肽聚糖得率15.8%。通过多重比较,最终确定采用三氯乙酸法提取嗜酸乳杆菌细胞壁肽聚糖。

Peptidoglycan in Lactobacillus acidophilus cell wall was extracted and purified by ultrasonic wave method, lysozyme method and trichloroacetic method. The optimal conditions for ultrasonic wave were ultrasonic power 400 W, ultrasonic time 30 min, ultrasonic temperature 40℃, yield rate of peptido-glycan was 10.2%; the optimal conditions for lysozyme method were lysozyme 4 mg/mL, 55℃, 5 h,pH 7, yield rate of peptidoglyean was 10.0% ; yield rate of peptidoglycan by trichloroacetic method was 15.8%. So trichloroacetic method was selected to extract peptidoglycan in Lactobacillus acidophilus cell wall.