小鼠胚胎干细胞核心转录因子nanog靶基因Zbtb9的生物信息学分析

Bioinformaton Analysis of Zbtb9 Gene of Mouse Embryonic Stem Cells Transcription Factor Nanog Downstream

通过特异性引物用RT-PCR方法扩增Zbtb9基因。利用生物信息学对小鼠的Zbtb9基因启动子进行生物信息学分析,预测Zbtb9基因启动子位置和启动子区内含有的转录因子结合位点,以及nanog在Zbtb9基因转录调控中的作用及Zbtb9蛋白的基本性质。Zbtb9基因启动子区可能定位于转录起始点上游790 bp至1024 bp之间。启动子区内含有15个转录调控因子结合位点,nanog的作用靶序列位于Zbtb9基因启动子区与转录起始位点之间,提示nanog在Zbtb9基因转录调控中起作用,小鼠Zbtb9蛋白二级结构可能以螺旋为主,富含疏水性氨基酸;对Zbtb9基因疏水区的氨基酸进行跨膜区分析,提示可能是跨膜蛋白。经SMART分析,Zbtb9蛋白含有BTB/POZ结构域。

Zbtb9 gene was amplified by reverse transcription-polymerase chain reaction（RT-PCR） using the special primers.To analyze the Zbtb9 genes using bioinformatics tools and predict the promoter position and transcription factor binding sites Methods.Predict the function of nanog in the Zbtb9 genes transcription regulation.Predict the Zbtb9 protein by bioinformatic methods.The promoter of zbtb9 was located in 790-1024bp of up-steam of transcriptional site.Transcription factor binding sites of zbtb9 was found with 15 transcription factors.The nanog taget sequence located between promoter region and transcriptional site showed that nanog play an important role in Zbtb9 genes transcription regulation.The transmembrane analysis of the amino acids demonstrated that the secondary structure was helix and this protein contain a transmembrane domain mainly could be transmembrane protein.Zbtb9 cotain BTB/POZ domain by SMART analying.