冬青卫矛内生真菌代谢产物农用杀菌活性筛选

Screening of Pesticidal Activities of Plant Endophytic Fungi from Euonymus japonicus

从冬青卫矛(Euonymus japonicus)中分离到59株植物内生真菌,农用杀菌活性测定表明,M-1菌株发酵液对番茄早疫病菌(Alernaria solani)、西瓜枯萎病菌(Fusarium oxysporium)和苹果炭疽病菌(Colleto-trichum gloesporioides)菌丝生长抑制率达到80.3%、78.5%和87.8%。M-1发酵液对枯草芽孢杆菌、蜡状芽孢杆菌、金黄色葡萄球菌和大肠杆菌的抑菌圈直径分别为23.0 mm、20.0 mm、16.0 mm和20.0 mm。M-1发酵液的乙酸乙酯萃取物对苹果炭疽病菌的毒力最高,IC50为63.6μg/mL。Doskochilova溶剂系统鉴定表明,M-1的发酵液中含有硫藤黄菌素类抗生素。

59 strains plant endophytic fungi were separated from Euonymus japonicus for antifungal activities screening,and got strain M-1 with strong antifungal bioactivities.The inhibition rate of metabolites of M-1 strain against Alternaria solani,Fusarium oxysporium sp.niveum,Colletotrichum gloesporioides were 80.3%,78.5% and 87.8%.The result of antibacterial bioassay indicated that the inhabitation zone of M-1 strain against Bacillus cereus,Bcillus subtills,Staphyococcus aureus and Escherichia coli were 23.0 mm,20.0 mm,16.0 mm and 20.0 mm.The ethyl acetate extract of M-1 broth culture had higher antifungal activity against Colletotrichum gloesporioides,and the IC50 was 63.6 μg/mL.By the Doskochilova solvent system,fermentation broth of M-1 may include sulfurluteolin.