TAT-Flip融合蛋白在小鼠大脑中动脉梗死模型中的保护作用

The neuroprotection of fusion protein TAT-Flip in MCAO mice models

目的探讨TAT-Flip融合蛋白在小鼠大脑中动脉梗死模型中的保护作用及其机制。方法制备小鼠大脑中动脉梗死模型,分别检测对照组、90min大脑中动脉梗死(MCAO)组、PBSMCAO组、FlipMCAO组、TAT-FlipMCAO组梗死体积的变化,应用Western印迹法观察caspase-8,caspase-3,Bcl-xl蛋白表达的变化。结果TAT-Flip融合蛋白可以减少梗死面积,引起caspase-8,caspase-3的蛋白表达下降,Bcl-xl蛋白表达升高。结论TAT-Flip融合蛋白可以减少小鼠MCAO模型的梗死面积,保护脑组织,减少脑损伤。

Objective To study the neuroprotection of TAT-Flip in middle cerebral artery occlusion （MCAO） mice models. Meth. ods 50 mice were divided into 5 groups： control, 90 rain MCAO, PBS MCAO and Flip MCAO and TAT-Flip MCAO groups to observe infarction volume and determine the protein expression of caspase 8, 3 and Bcl-xl. Results TAT-Flip MCAO group had decreased infarction volume, the reduced protein expressions of caspase 8 and 3 and the increased Bcl-xl protein expression. Conclusions TAT-Flip could lessen brain injury, playing a neuroprotective role in MCAO mice models.