贝那普利对兔急性心肌梗死再灌注后内皮素-1及无复流的影响

Effects of benazepril on endothelin-1 and myocardial no-reflow in a rabbit model of acute myocardial infarction and reperfusion

目的评价贝那普利对兔急性心肌梗死(AMI)再灌注后内皮素-1(endothelin-1,ET-1)的影响及对无复流的防治作用。方法新西兰大白兔24只随机分成对照组、贝那普利组[3 mg/(kg.d)]和假手术组,每组8只。将冠状动脉结扎60 min、松解120 min,制备AMI再灌注模型。梗死前、后和再灌注后,用导管法进行血液动力学测定。采用放射免疫测定法检测血浆及心肌组织中ET-1的水平,最终进行病理学分析。结果与对照组相比,贝那普利组能明显降低兔AMI再灌注后血浆ET-1的水平(P<0.01);而心肌组织中ET-1的含量仅复流区显著降低(P<0.01)。与对照组相比,贝那普利可促进AMI后心功能的恢复,减少无复流的面积[对照组为75.3%结扎区(LA),贝那普利组为35.9%LA,P<0.01]。结论贝那普利能有效降低血浆及心肌组织中ET-1的水平,具有内皮保护作用;能有效地防治心肌梗死再灌注后无复流现象的发生。

AIM To evaluate the effects of benazepril on myocardial no-reflow in a rabbit model of acute myocardial infarction and reperfusion. METHODS Twenty-four rabbits were divided randomly into 3 groups （ 8 each group）, namely, control group, benazepril 13 mg/（ kg·d） 1 group and sham operation group. The animals in the former two groups were subjected to a 60 minute coronary occlusion, followed by a 120 minute reperfusion. Data on haemodynamics were collected and the levels of ET-1 in blood samples and myoeardium were determined by radioimmunoassay （RIA）. The areas of no-reflow were evaluated with pathological means. RESULTS In benazepril group, the levels of plasma ET-1 were significantly lower than those in the control group （P 〈0. 01 ）, whereas the level of ET-1 in infarcted reflow myocardium was significantly lower than that in the control group （P 〈 0.01 ）. In the control group, the area of no-reflow was 75.3% of ligation area（LA） , whereas the area was significantly decreased to 35.9% of LA in benazepril group （P 〈0. 01 ）. CONCLUSION Benazepril is effective in reducing ET-1 in blood plasma and local myocardium and protecting endothelial cells. Benazepril also has some beneficial effects on preventing myocardial no-reflow, improving left ventrieular function and reducing infarcted area during acute myocardial infarction and reperfusion in rabbits.