人牙周膜干细胞雌激素受体表达检测的实验研究

Study on estrogen receptor expression in human periodontal ligament stem cells

目的:检测体外分离纯化的人牙周膜干细胞(PDLSCs)是否有雌激素受体(ER)表达。方法:采用免疫细胞化学染色、Western blot、RT-PCR技术检测PDLSCs中雌激素受体ER-α、ER-β的表达。结果:免疫细胞化学检测显示ER-α、ER-β在牙周膜干细胞内均呈棕黄色阳性染色,定位于细胞核;Western blot与RT-PCR测定结果显示,PDLSCs中ER-α及ER-β表达高于相同来源的牙周膜细胞,PDLSCs中ER-α表达高于ER-β,且在雌激素作用下ER-α及ER-β表达均增加。结论:体外分离纯化的PDLSCs中存在雌激素受体(ERα、ERβ)的表达,且受雌激素的调控。

AIM ： To detect the expression of estrogen receptors （ERs） in isolated human periodontal ligament stem cells（PDLSCs）. METHODS： Immunocytochemistry, Western blot analysis and RT- PCR procedure were used to detect ER - α and ER - β expression in PDLSCs. RESULTS ： Immunocytochemistry showed that both ER - α and ER - α were positively stained in the nuclei of PDLSCs. Western blot analysis and RT - PCR procedure indicated that the expressions of ERs （ both ER - α and ER - β） in PDL cells were in a lower level compared with those in the PDLSCs. In PDLSCs groups, the expression of ER - α was higher than those of ER - β, and the expressions of ERs in 17 - β estradiol treated group were higher than those of untreated group. CONCLUSION： Both ER - α and ER - β are expressed in PDLSCs, and the expression is up - regulated by estrogen in vitro.