肿瘤转移抑制因子TXNIP基因与精神分裂症易感性研究

The study of susceptibility to metastasis suppressor TXNIP gene in schizophrenia.

目的研究肿瘤转移抑制因子TXNIP在精神分裂症患者和正常对照外周血白细胞中的表达水平,分析该基因与疾病的关系。方法采用TaqMan MGB探针法的绝对定量PCR分析30例首发未服用抗精神病药物、30例复发服用抗精神病药物治疗的精神分裂症患者及30名正常对照TXNIP基因的表达水平。采用阳性和阴性症状量表(PANSS)评定患者病情并由此将患者组分为不同亚组,比较各患者组和对照组间TXNIP基因表达的差异。结果①总患者组TXNIP基因的平均表达水平比对照组低30.2%(P=0.03),而复发服药患者组比对照组低94.0%(P<0.01),首发未服药组比对照组高33.6%(P<0.01)。②未发现各患者组TXNIP基因表达量与PANSS评分、服药剂量相关。③复发服药患者中有家族史的患者TXNIP基因的表达量低于无家族史患者(P=0.01)。结论提示TXNIP基因可能是精神分裂症新的候选基因,抗精神病药物可能降低TXNIP基因的表达。

Objective To investigate the expression of TXNIP gene in peripheral leucocytes from patients with schizophrenia. Methods We measured expression levels of TXNIP in the leukocytes from 30 recurrent patients taking antipsychotic medicine, 30 first-episode drug-nai＇ve patients, and 30 healthy controls using absolute TaqMan real-time quantitative PCR. Further, the two ease groups were divided into different subgroups using Positive and Negative Syndrome Scale （PANSS） respectively and then assessed the expression of TXNIP gene among different subgroups. Results ① The average expression level of TXNIP in leucocytes from total patients with schizophrenia was significantly lower than that of healthy controls by 30. 2% （ P = 0. 03 ）. That of the recurrent taking antipsychotie medicine group was much lower than that of healthy control group by 94. 0% （ P 〈 0. 01 ）, while that of the first-episode drug-naive group was higher than that of healthy control group by 33.6% （ P 〈 0.01 ） ; ②There was no significant correlation between the expression of TXNIP and the scores of PANSS and the dose of antipsychotic medicines. ③ The average expression of TXNIP in patients with family history of schizophrenia was significant lower than that of without family history （P = 0. 01 ）. Conclusions The results suggest that TXNIP might be a novel candidate gene conferring susceptibility to schizophrenia and antipsychotie medicines might decrease the expression of TXNIP gene.