槲皮素对大鼠癫痫持续状态后海马XIAP mRNA与蛋白表达的影响

The expression of XIAP mRNA and protein in the rat hippocampus and quercetin treatment following status epilepticus.

目的研究大鼠癫痫持续状态（SE）后海马组织XIAP的表达变化及槲皮素对其表达的影响。方法建立氯化锂-匹罗卡品致痫大鼠sE模型，应用免疫组化和RT-PCR方法检测XIAP与caspase-3蛋白以及XIAPmRNA的表达。结果海马CA3区XIAP蛋白在SE后2h（0．5503±0．0172）起逐渐增加，8h（0．6221±0．0238）达高峰，与对照组比较（0．1507±0．0165），差异有统计学意义（P〈0．01）。海马CA3区caspase-3活性蛋白在对照组未见明显表达，在SE后4—72h明显增多。与对照组比较，SE组海马XIAPmRNA表达水平在2～8h增加（P〈0．01）。与SE组比较，槲皮素组海马XIAPmRNA表达水平及CA3区XIAP蛋白表达在8h、24h高于SE组（P〈0．01），CA3区caspase-3活性蛋白表达在8h、24h、72h低于SE组（P〈0．01）。结论XIAP可能参与了SE后神经元凋亡的调控过程，槲皮素可以提高SE后海马神经元XIAP的表达。

Objective To investigate the expression of XIAP mRNA and protein in the rat hippocampus and quercetin treatment following status epilepticus （SE）. Methods The lithium-pilocapine model of status epilepticus was established in SD rat. XIAP and activated caspase-3 protein were examined using immunohistochemistry. XIAP mRNA were ex- amined using semiquantitative RT-PCR. Results Compared with the control group, the expression of CA3 XIAP protein in the SE group was increased gradually since 2 h（0. 5503 ±0. 0172 vs 0. 1507 ±0. 0165, P 〈0. 01 ）, and reached a peak at 8 h（0. 6221 ±0. 0238 vs 0. 1507 ±0. 0165, P 〈0. 01 ）. The expression of CA3 activated caspase-3 protein were increased generally following SE. Compared with the control group, the XIAP mRNA levels revealed increase at 2 h, 4 h and 8 h （P 〈0. 01 ） in the SE group. Compared with the SE group, theexpression of XIAP mRNA and protein was increased at 8 h and 24 h （P 〈0. 01 ） in quercetin group, but the expression of CA3 activated caspase-3 protein revealed decrease in the quercetin group at 8 h, 24 h and 72 h （ P 〈 0. 01 ）. Conclusions The XIAP are involved in the regulation of neuronal apoptosis after SE. Quercetin may increase the expression of XIAP in the rat hippocampus following SE.