东亚对开蕨离体快繁的研究

Studies on in Vitro Propagation of Endangered Plant Phyllitis japonia

利用东亚对开蕨(Phyllitis japonica Kom.)孢子叶为外植体,进行了组培快繁的研究。结果表明:不同接种方式、不同的培养方法对东亚对开蕨孢子萌发和原叶体生长均有明显影响,破碎孢子囊后的孢子以及液体培养中的孢子萌发较快;原叶体在无植物生长调节剂的MS培养基中以5倍的速率增殖;喷施0.1%KH2PO4溶液的试管外培养更适于原叶体向孢子体植株的转化。

The sporophyllary leaves of Phyllitis japonica were used as explants to study the process of tissue culture rapid reproduction. The results showed that different inoculation types and culture methods significantly affected the spores germination and prothallus growth of Phyllitis japonica. The spores which sporangia were broken and cultured in liquid culture media germinated quickly. The prothallus propagated in MS culture medium without the plant growth hormone at a speed rate of 5 times. Pot culture of spraying 0. 1% KH2PO4 upon prothallus is more suitable to translated from prothallus to spore.